Open AccessAmplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
Author(s) -
Shimada Masamitsu,
Fukushima Michio,
Mukai Hiroyuki,
Kato Ikunoshin,
Nishikawa Akira,
Fujinaga Kei
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02498.x
Subject(s) - polymerase chain reaction , microbiology and biotechnology , oligonucleotide , human papillomavirus , biology , oligomer restriction , carcinoma , cervical carcinoma , gene , gel electrophoresis , papillomaviridae , virology , cervical cancer , cancer , genetics , medicine
We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use of a specific oligonucleotide probe. The PCR could detect 10 ‐5 –10 ‐6 copies per cell (maximum sensitivity). Furthermore, HPV 16, 18 and 33 DNAs were synthesized in a common reaction solution and specifically detected by HPV type‐specific probes. The PCR detected the HPV sequence from tissues which were negative to Southern hybridization. This detection technique may contribute significantly to the precise analysis of HPV in small proliferative lesions in the cervix.