Open AccessHigh Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
Author(s) -
Urade Masahiro,
Sugi Masakazu,
Mima Takashi,
Ogura Takafumi,
Matsuya Tokuzo
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02337.x
Subject(s) - hela , bleomycin , microbiology and biotechnology , poly adp ribose polymerase , cell culture , nicotinamide , enzyme , biochemistry , biology , chemistry , polymerase , cell , chemotherapy , genetics
Poly(ADP‐ribose) polymerase activity was measured in bleomycin (BLM) ‐ resistant HeLa (HeLa‐BLM r ) and the parental HeLa cells after BLM treatment. HeLa‐BLM r cells, which had been subcultured in growth medium containing 1 μg/ml of BLM, showed a 3.75‐fold higher enzyme activity than did HeLa cells, but this activity was decreased to the same level as that of HeLa cells after 48 h of BLM‐free cultivation. When HeLa and HeLa‐BLM r cells after a 48‐h cultivation in BLM‐free growth medium were treated with BLM, the enzyme activity was induced at a higher level (2.5–3.8 times) in HeLa‐BLM r than in HeLa cells and was inhibited markedly in HeLa‐BLM r and slightly in HeLa cells by nicotinamide, an inhibitor of this enzyme. The BLM‐induced cell killing by nicotinamide was highly potentiated (about 18 times) in HeLa‐BLM r as compared to HeLa cells.