Open AccessEndothelin Potentiates Growth Factor‐stimulated DNA Synthesis in Swiss 3T3 Cells
Author(s) -
Kusuhara Masatoshi,
Yamaguchi Ken,
Ohnishi Ai,
Abe Kaoru,
Kimura Satoshi,
Oono Hideto,
Hori Shingo,
Nakamura Yoshiro
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02310.x
Subject(s) - dna synthesis , endothelin receptor , endothelin 1 , endocrinology , growth factor , endothelins , medicine , cell culture , biology , vasoconstriction , microbiology and biotechnology , basic fibroblast growth factor , intracellular , cell growth , fibroblast growth factor , 3t3 cells , receptor , biochemistry , dna , transfection , genetics
A murine cell line, Swiss 3T3, is commonly used for the study of cellular growth. The present study revealed that this cell line possesses high‐affinity receptors for endothelin, a vasoactive peptide derived from vascular endothelial cells. In this cell line, endothelin potentiated DNA synthesis stimulated by platelet‐derived growth factor, basic fibroblast growth factor or insulin. The endothelin concentration required for potentiating DNA synthesis in this cell line is almost the same as that reported previously in endothelin‐induced vasoconstriction. Since endothelin increased intracellular calcium levels, this ion may participate in the signal transduction pathways by which endothelin induces potentiation of DNA synthesis.