Open AccessCoamplification of the L‐ myc and N‐ myc Oncogenes in a Neuroblastoma Cell Line
Author(s) -
Jinbo Takeshi,
Iwamura Yukio,
Kaneko Michio,
Sawaguchi Shigenori
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02309.x
Subject(s) - n myc , neuroblastoma , gene duplication , cell culture , gene , biology , microbiology and biotechnology , southern blot , oncogene , cancer research , gene expression , genetics , cell cycle , ganglioneuroma
The L‐ myc , N‐ myc and c‐ myc genes are members of the myc oncogene family. In particular, L‐ myc is novel, and amplification of L‐ tnyc is still unknown except in small cell lung carcinoma. We examined L‐ myc amplification in 30 human neuroblastomas using Southern blot hybridization, and found that the L‐ myc gene was amplified approximately 5‐fold in GOTO, a human neuroblastoma cell line. The N‐ myc gene was also amplified approximately 60‐fold and furthermore, over‐expression of L‐ myc and N‐ myc genes was observed in this cell line. In this report, we describe the coampliflcation of the myc gene family in the GOTO neuroblastoma cell line.