Open AccessHematoporphyrin as a Sensitizer of Cell‐damaging Effect of Ultrasound
Author(s) -
Yumita Nagahiko,
Nishigaki Ryuichiro,
Umemura Koshiro,
Umemura Shinichiro
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02295.x
Subject(s) - hematoporphyrin , trypan blue , sarcoma , in vitro , microbiology and biotechnology , pathology , cell , chemistry , tumor cells , medicine , biology , cancer research , biochemistry , photodynamic therapy , organic chemistry
Mouse sarcoma 180 or rat ascites hepatoma (AH) 130 cells were exposed to ultrasound (US; 1.27, 2.21 and 3.18 W/cm 2 ; 1.92 MHz) for up to 60 s in vitro in the presence or absence of hematoporphyrin (Hp; 10, 25 and 50 μg/ml). The cell‐damaging effects of treatments were determined by means of the Trypan Blue dye exclusion test. Hp alone did not show any cell‐damaging effect, whereas US alone damaged 30 and 50% of sarcoma and AH 130 cells, respectively, at the maximum intensity for 60 s. In the presence of 50 μg/ml Hp, US damaged 99 and 95% of the above tumor cells, respectively. These results show that Hp increased the sensitivity of tumor cells to US.