Open AccessLung Cancer Cells Often Express High Levels of Protein Kinase C Activity
Author(s) -
Hirai Masamichi,
Gamou Shinobu,
Kobayashi Makoto,
Shimizu Nobuyoshi
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02292.x
Subject(s) - protein kinase c , phosphatidylserine , lung cancer , cancer , tetradecanoylphorbol acetate , cancer cell , lung , biology , cell culture , kinase , stomach , cancer research , endocrinology , medicine , pathology , chemistry , microbiology and biotechnology , biochemistry , phospholipid , genetics , membrane
We analyzed protein kinase C (PKC) activity in twenty‐two tumor cell lines derived from lung, pancreas, stomach, tongue and vulva, and found that lung cancer cells often (9 out of 13) exhibit significantly higher PKC activity than other types of cancer cells. The PKC in these lung cancer cells was separated into one major and one minor peaks by a Mono Q column chromatography. The PKC in the major peak had an absolute requirement for Ca 2± , phosphatidylserine and 12‐O‐tetradeca‐noylphorbol‐13‐acetate (TPA), as expected. However, the PKC in the minor peak did not require TPA for its activation. Hydroxyapatite column chromatography revealed that the PKC in the major peak is type III. These results indicate that in lung cancer cells type III PKC activity is often elevated compared to other types of cancer cells. The growth of many lung cancer cell lines was inhibited by TPA.