Open AccessConstruction of Mouse A9 Clones Containing a Single Human Chromosome (X/Autosome Translocation) via Micro‐cell Fusion
Author(s) -
Koi Minoru,
Morita Hiroyuki,
Shimizu Motoyuki,
Oshimura Mitsuo
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02278.x
Subject(s) - chromosomal translocation , biology , microbiology and biotechnology , karyotype , hypoxanthine guanine phosphoribosyltransferase , chimera (genetics) , cell fusion , autosome , genetics , chromosome , cell , gene , mutant
Cell hybrids between hypoxanthine guanine phosphoribosyl transferase (HGPRT)‐deficient mouse cell lines (A9 or RAG) and each of 12 different human flbroblasts (GM cells) containing various X/autosome translocations were formed, selected and isolated. Several human chromosomes including an X/autosome translocation carrying HGPRT locus were found in these hybrid cells. To construct A9 cell clones that contain a single X/autosome translocation, micro‐cell fusion was undertaken to transfer these chromosomes from the hybrids to A9 cells. Karyotype analysis revealed that most of the resulting micro‐cell hybrids contain, in a background of mouse chromosomes, only the human X/autosome translocations which were present in the GM cells used for cell hybridization. Sublines of A9 cells were established containing the following autosomal segments: 1q23→1qter; 1q12→1pter; 3p12→3pter; 3q21→3qter; 11q13→11qter; 11q13→11pter; 11p11→11qter; 11q23→11pter; 12q24→12pter; 16q24→16pter; 17q11→17pter.