Open AccessEffect of Human Interferons on Morphological Differentiation and Suppression of N‐ myc Gene Expression in Human Neuroblastoma Cells
Author(s) -
Watanabe Hisao,
Chisaka Takeshi,
Higuchi Takakazu,
Tanaka Atsuo,
Horii Yoshihiro,
Sugimoto Tohru,
Imanishi Jiro
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02261.x
Subject(s) - neuroblastoma , biology , gene expression , neurite , cycloheximide , microbiology and biotechnology , recombinant dna , cellular differentiation , gene , cancer research , cell culture , protein biosynthesis , in vitro , biochemistry , genetics
The activity of human interferons (HuIFNs) to induce morphological changes and the suppression of N‐ myc gene expression on human neuroblastoma cells (GOTO and KP‐N‐RT) was evaluated. Morphological differentiation, characterized as the extension and bifurcation of neurites, the formation of multinucleated giant cells and the formation of neurite networks, was induced by treatment with recombinant HuIFN‐γ (rHuIFN‐γ and also with natural HuIFN‐γ on human neuroblastoma cells (GOTO and KP‐N‐RT). But recombinant HuIFN‐αA and recombinant HuIFN‐βdid not induce any changes. The rHuIFN‐β and rHuIFN‐γ inhibited the growth of GOTO and KP‐N‐RT cells more strongly than the rHuIFN‐αA did. The expression of N‐ myc gene was suppressed in GOTO cells treated with rHuIFN‐γ. The suppressive effect of rHuIFN‐γ was dependent on the duration of the treatment. However, rHuIFN‐αA and rHuIFN‐β did not suppress N‐ myc gene expression. Moreover, both morphological differentiation and the supprcssive effect on N‐ myc gene expression by rHuIFN‐γ were inhibited in the presence of cycloheximide. These results suggest that the morphological changes and N‐ myc gene expression in neuroblastoma cells are closely related. Furthermore, this decreased N‐ myc gene expression during the morphological differentiation may be related to the proteins induced by HuIFN‐γ.