Open AccessAccumulation of Abnormally High Ploid Nuclei in the Liver of LEC Rats Developing Spontaneous Hepatitis
Author(s) -
Fujimoto Yoshinori,
Oyamada Masahito,
Hattori Atsuo,
Takahashi Hidetoshi,
Sawaki Masakuni,
Dempo Kimimaro,
Mori Michio,
Nagao Minako
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02243.x
Subject(s) - jaundice , hepatitis , biology , binucleated cells , polyploid , hepatocyte , deoxyuridine , medicine , endocrinology , pathology , microbiology and biotechnology , immunology , dna , genetics , ploidy , toxicity , micronucleus , micronucleus test , gene , in vitro
Enlarged hepatocytes with huge nuclei were found in LEC rats with hereditary hepatitis. Flow cytometric analysis of the DNA content of nuclei from jaundiced LEC rats revealed the presence of very high polyploids, such as 32n and 64n. At the age of 12 weeks, before the onset of hepatitis, 8n polyploid nuclei were more frequent in LEC rats than in LEA rats, a sibling line of LEC rats. Binucleated hepatocytes were also more frequent in LEC rats than in LEA rats at week 4. Bi‐, tri‐ and tetra‐nucleated cells whose nuclei were sometimes different in size were observed when jaundice became manifest. The number of proliferating liver cells, determined by pulse labeling with 5‐bromo‐ 2′‐deoxyuridine (BrdU), was higher in LEC rats than in LEA rats at 2, 4, 8, 12 and 14 weeks, with a maximum at week 4. A remarkable increase of BrdU uptake was observed at week 16, when jaundice developed. The possible involvement of abnormal cytokinesis and kariokinesis in the manifestation of hepatitis was suggested.