Open AccessA Murine Monoclonal Antibody that Recognizes an Extracellular Domain of the Human c‐erbB‐2 Protooncogene Product
Author(s) -
Masuko Takashi,
Sugahara Kunio,
Kozono Mitsuko,
Otsuki Seiko,
Akiyama Tetsu,
Yamamoto Tadashi,
Toyoshima Kumao,
Hashimoto Yoshiyuki
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02237.x
Subject(s) - transfection , gene product , microbiology and biotechnology , 3t3 cells , biology , cell culture , monoclonal antibody , extracellular , erbb , antibody , gene , gene expression , biochemistry , immunology , genetics
A marine IgM monoclonal antibody, designated SV2–61, was generated against human c‐erbB‐2 gene‐transfected NIH‐3T3 (SV11) cells. SV2–61 defined a 185‐kDa molecule present on the surface of SV11 cells, another line of c‐erbB‐2 gene‐transfected NIH‐3T3 (A4–15) cells, and MKN‐7 human gastric cancer cell line carrying an amplified human c‐erbB‐2 gene. The SV2–61‐defined antigen was found to show protein kinase activity in vitro . The SV2–61 was reactive with human c‐erbB‐2 gene‐transfected NIH‐3T3 cell lines but not with transfectants carrying c‐erbB‐2 gene mutants which lack a coding region for the extracellular domain. It was reactive with a portion of human epithelial cell lines hut not with native NIH‐3T3, TGF‐a‐coding gene‐, activated c‐raf gene‐ or Ha‐ras gene‐transfected NIH‐3T3 cells, or non‐epithelial human cells. These results indicate that the SV2–61 is an antibody which recognizes an extracellular domain of the c‐erbB‐2 gene product, 185‐kDa protein.