Open AccessAmplification of Both c‐myc and c‐raf ‐1 Oncogenes in a Human Osteosarcoma
Author(s) -
Ikeda Shogo,
Sumii Hiroshi,
Akiyama Kosuke,
Watanabe Sekiko,
Ito Shiro,
Inoue Hajime,
Takechi Hideo,
Tanabe Gozo,
Oda Takuzo
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb02236.x
Subject(s) - osteosarcoma , gene duplication , southern blot , oncogene , gene , microbiology and biotechnology , biology , cancer research , proto oncogenes , pathology , medicine , genetics , cell cycle
Fourteen human bone and soft part tumor tissues were screened by Southern blot hybridization using five oncogene probes (c‐ myc , c‐K‐ ras , c‐ fos , c‐ raf ‐l, and N‐ myc ). Amplification of c‐ myc was found in two osteosarcomas and one malignant fibrous histiocytoma. One of these osteosarcomas had amplified c‐ raf ‐1 gene. Rearrangement of the amplified gene was not observed. This is the first report of c‐ raf ‐1 amplification in human cancer tissues.