Open AccessIn situ Freezing of the Rat Urinary Bladder: DNA Adduct Formation in the Bladder Epithelium Demonstrated by 32 P‐Postlabeling Assay
Author(s) -
Hasegawa Ryohei,
Yamashita Katsumi,
Morimoto Kazushige,
Furukawa Fumio,
Toyoda Kazuhiro,
Shimoji Naoshi,
Takahashi Michihito,
Hayashi Yuzo
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb01722.x
Subject(s) - epithelium , in situ , adduct , urinary system , urinary bladder , dna , urothelium , microbiology and biotechnology , chemistry , pathology , urology , medicine , biology , biochemistry , organic chemistry
In situ freezing of the urinary bladder has been demonstrated to exert tumor‐initiating potential in two‐stage urinary bladder carcinogenesis in the rat. In the present experiment, DNA modification was examined after in situ freezing of the whole urinary bladder performed by pinching with frozen forceps at —15°C or —30°C for 2 s. The 32 P‐postlabeling analysis revealed at least 2 DNA adducts in the epithelial cells of the urinary bladder collected 3 days after freezing. Single‐strand breaks of DNA were also found by means of the alkaline elution assay in the bladder epithelium collected 10 min after freezing. Thus, the previously demonstrated tumor‐initiating activity of in situ freezing in urinary bladder carcinogenesis was revealed to be associated with substantial DNA damage and adduct formation.