Open AccessThe × Gene of Hepatitis B Virus Induced Growth Stimulation and Tumorigenic Transformation of Mouse NIH3T3 Cells
Author(s) -
Shirakata Yumiko,
Kawada Minako,
Fujiki Yukio,
Sano Haruhiko,
Oda Munehiro,
Yaginuma Katsuyuki,
Kobayashi Midori,
Koike Katsuro
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb01686.x
Subject(s) - hbx , carcinogenesis , gene , gene product , hepatitis b virus , biology , messenger rna , cell culture , microbiology and biotechnology , coding region , virology , virus , gene expression , cancer research , malignant transformation , cell growth , genetics
To examine the transforming potential of the × gene product of hepatitis B virus (HBV), the X‐gene‐containing region (referred to as the HBx region) was introduced into mouse NIH3T3 cells. Each transformed cell line expressed X‐coding mRNA at a different level. A positive correlation was found between the level of X‐coding mRNA and the saturation density of the cells. The HBx‐transformed cell lines exhibited × protein production and tumor formation in nude mice. The function of HBV in oncogenesis may involve the continuous expression of the X‐gene‐coded product in the HBV DNA‐integrated cells.