Open AccessEnhanced ATPase Activity in Liver Cell Nuclei Induced by Administration of Mitomycin C to Rats
Author(s) -
Wakizaka Akira,
Imai Hirokazu,
Aiba Namiko,
Okuhara Eiji
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb01656.x
Subject(s) - ouabain , nucleoside triphosphate , atpase , mitomycin c , biochemistry , enzyme , triphosphatase , chemistry , nucleoside , microbiology and biotechnology , biology , sodium , nucleotide , genetics , organic chemistry , gene
Intraperitoneal administration of mitomycin C (40 μg/100 g body weight) to male Wistar rats increased the ATPase activity in hypotonic extracts of liver cell nuclei for 4 days after injection. Partially purified ATPase, obtained by the DEAE‐cellnlose column chromatography of these extracts, showed a 14 times higher specific activity than that found in normal rat liver nuclei. The enzymatic activity was strongly enhanced by the addition of polynucleotides, especially poly A and poly I, to the assay mixture, but was inhibited by GTP, a chelating agent, heparin and thiol‐group inhibitors. Quercetin and oligomycin were less effective, and ouabain showed no inhibitory effect. Mg 2+ ions were essential, but neither Ca 2+ , Na + nor K + ions were required for the manifestation of the activity. These characteristic properties of the enzyme are similar to those of a nucleoside triphosphatase found in the nuclear matrix and envelope, suggesting that some energy‐providing mechanisms involved in the repair processes of DNA damage or cellular injury are induced by mitomycin C administration.