Open AccessSecretion of Transforming Growth Factor‐ß by Human Myelogenous Leukemic Cells and Its Possible Role in Proliferation of the Leukemic Cells
Author(s) -
Komatsu Keiko,
Nakamura Hiroyuki,
Shinkai Kiyoko,
Akedo Hitoshi
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb01628.x
Subject(s) - k562 cells , pronase , paracrine signalling , transforming growth factor , secretion , growth factor , biology , cell culture , 3t3 cells , chronic myelogenous leukemia , cell growth , leukemia , transforming growth factor beta , microbiology and biotechnology , endocrinology , immunology , biochemistry , trypsin , transfection , genetics , receptor , enzyme
Transforming growth factor(TGF)‐ß activity was found in the neutral extracts of human myelogenous leukemic cells or K562 cells and the conditioned medium from K562 cell culture. BALB/c 3T3 cells grown in soft agar in the presence of TGF‐ß 1 produced an activity that stimulated the growth of K562 cells. This activity was non‐dialyzable. acid‐stable, heat‐sensitive and partially Inactivated by pronase treatment. These results suggest a mutual growth reliance between the leukemic cells and fibroblasts mediated by paracrine growth factors produced by these cells.