Open AccessNucleotide Sequence and Chromosomal Mapping of the Human c‐ yes ‐2 Gene
Author(s) -
Semba Kentaro,
Nishizawa Makoto,
Satoh Hitoshi,
Fukushige Shinichi,
Yoshida Michihiro C.,
Sasaki Motomichi,
Marsusbra Kenichi,
Yamamoto Tadashi,
Toyoshima Kumao
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb02227.x
Subject(s) - pseudogene , gene , genetics , biology , chromosome , nucleic acid sequence , gene mapping , metaphase , human genome , chromosome 22 , genome , sequence (biology) , chromosome instability , microbiology and biotechnology
We molecularly characterized the second gene, c‐ yes ‐2, of two copies of yes ‐related genes which we previously found to contain in the human genome. First, nucleotide sequence analysis revealed that the c‐ yes ‐2 gene is a pseudogene of the c‐ yes ‐1 gene. Second, by using two independent methods, hybridization of both DNAs from sorted chromosomes and metaphase spreads with c‐ yes ‐2 DNA, we assigned the c‐ yes ‐2 gene to chromosome 22q11.2. This chromosomal localization is inconsistent with that given in our previous report. The failure of proper mapping in our experiment might have been caused by instability of hybrid cell clones.