Open AccessAMPLIFIED ALLELE OF THE HUMAN N‐ myc ONCOGENE IN NEUROBLASTOMAS
Author(s) -
Yamada Masao,
Kurosawa Hidemitsu,
Nakagawa Yuichi,
Nakagome Yasuo,
Tsuda Tohru,
Higashi Ken
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb02219.x
Subject(s) - allele , biology , microbiology and biotechnology , haplotype , oncogene , genetics , restriction fragment length polymorphism , intron , polymerase chain reaction , dna , gene , cell cycle
The N‐ myc amplification unit was investigated using two restriction fragment length polymorphisms for SphI and PvuII which we previously found to occur in the 2nd intron and in the 3’region of the human N‐ myc oncogene, respectively. All the three haplotypes which are dominant in the Japanese population were observed in the amplified DNA after analyses of a total of 22 DNA samples of fresh neuroblastomas with N‐ myc amplification. We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.