Open AccessDIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
Author(s) -
Sato Chieko,
Miyaki Michiko,
Oikawa Shinzo,
Nakazato Hiroshi,
Kosaki Goro
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb01610.x
Subject(s) - carcinoembryonic antigen , microbiology and biotechnology , antigen , complementary dna , messenger rna , northern blot , biology , gene expression , colorectal cancer , gene , pathology , cancer , medicine , immunology , biochemistry , genetics
The level of mRNA for carcinoembryonic antigen (CEA) and nonspecific crossreacting antigen (NCA) in human colon adenocarcinomas and normal colon mucosa was analyzed by Northern blot hybridization using as probes 32 P‐labeled CEA cDNA and synthetic oligodeoxyribonucleotides specific to CEA and NCA mRNA sequences. The major 3.5‐kb mRNA and a minor 4.2‐kb mRNA are shown to be CEA‐specific and expressed in both tissues, albeit at slightly different degrees, suggesting that the expression of CEA is regulated posttranscriptionally. Another minor mRNA of 2.9 kb is NCA‐specific and expressed predominantly in cancerous tissues, suggesting its usefulness as a marker for colon cancer.