Open AccessEFFECT OF CULTURE SUPERNATANT OF MT‐2 CELLS ON HUMAN IMMUNODEFICIENCY VIRUS‐PRODUCING CELLS, MOLT‐4/HIV HTLV‐IIIB CELLS
Author(s) -
Matsuyama Toshifumi,
Hamamoto Yoshiaki,
Yoshida Tsutomu,
Kido Yasuji,
Kobayashi Susumu,
Kobayashi Nobuyuki,
Yamamoto Naoki
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb01571.x
Subject(s) - lymphotoxin , cytotoxic t cell , cell culture , virology , monoclonal antibody , antibody , virus , tumor necrosis factor alpha , lymphotoxin alpha , biology , microbiology and biotechnology , immunology , in vitro , biochemistry , genetics
The effect of culture supernatant of MT‐2 cells on human immunodeficiency virus (HIV)‐producing cells, MOLT‐4/HIV HTLV‐IIIB cells, was examined. As compared to the effect on MOLT‐4 cells, parent cells not infected with HIV, a selective cytotoxic/cytostatic effect on MOLT‐4/ HIV HTLV‐IIIB cells was observed 4 days after treatment with up to 640‐fold‐diluted MT‐2 supernatant. Furthermore, under similar conditions, a 2‐ to 6‐fold increase in the number of HIV particles was detected in the culture of MOLT‐4/HIV HTLV‐IIIB cells 6 hr after treatment. Complete blocking of these effects by anti‐lymphotoxin monoclonal antibody, but not by anti‐tumor necrosis factor antibody, indicates that these effects of MT‐2 supernatant on MOLT‐4/HIV HTLV‐IIIB cells are attributable to a lymphotoxin‐related cytotoxic factor.