Open AccessIsolation and Characterization of ras ‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
Author(s) -
Sasaki Kiyoshi,
Mizusawa Hiroshi,
Ishidate Motoi
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb00056.x
Subject(s) - transfection , 3t3 cells , oncogene , clone (java method) , carcinogenesis , microbiology and biotechnology , cell culture , biology , cell , phorbol , population , tetradecanoylphorbol acetate , transformation (genetics) , malignant transformation , cell cycle , cancer research , dna , biochemistry , medicine , signal transduction , gene , genetics , environmental health , protein kinase c
The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ ras ) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras ‐transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.