Open AccessThe Mechanism of Acquired Resistance to Cisplatin by a Human Ovarian Cancer Cell Line
Author(s) -
Kikuchi Yoshihiro,
Iwano Ichiro,
Miyauchi Munenori,
Kita Tsunekazu,
Oomori Keibun,
Kizawa Isao,
Sugita Michio,
Tenjin Yoshio
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb00033.x
Subject(s) - cisplatin , serous cystadenocarcinoma , cell culture , ovarian cancer , endocrinology , ovary , medicine , cancer research , biology , cancer cell , chemistry , chemotherapy , cancer , genetics
The present study was designed to elucidate the mechanism of resistance to cisplatin. A cisplatin‐resistant cell line (KFr) was established from KF cells derived from human serous cystadenocarcinoma of the ovary. The DNA histogram revealed an increase of S‐phase cells and a decrease of G 1 ‐phase cells in cultured KFr cells, compared to that in cultured KF cells. Although the cisplatin content in the KF cells incubated with cisplatin at 10 μg/ml increased in a time‐dependent manner, that in the KFr cells remained unchanged during the experimental period. When 0.5 mg of cisplatin was administered ip to nude mice with KF or KFr tumor, the cisplatin content in the KFr tumor was significantly lower than that in the KF tumor. The KFr cells showed a cross‐resistance to L‐phenylalanine mustard, while no cross‐resistance to vincristine or 5‐fluorouracil was observed. These findings suggest that the mechanism of cisplatin resistance in the KFr cells involves a decrease of cisplatin accumulation in the tumor cells.