Open AccessTumor Necrosis Factor‐inducing Activities of Lipid A Preparations from Pseudomonas diminuta and Pseudomonas vesicularis
Author(s) -
Arata Satoru,
Nakaya Kazuyasu,
Furuhashi Hisayoshi,
Nakamura Yasuharu,
Hirayama Tohru,
Mashimo Junichi,
Kasai Nobuhiko
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb00032.x
Subject(s) - lipid a , tumor necrosis factor alpha , pseudomonas , biochemistry , amide , fatty acid , pseudomonadaceae , biology , chemistry , bacteria , endocrinology , lipopolysaccharide , genetics
Tumor necrosis factor (TNP)‐inducing activities of lipid A preparations from P. diminuta and P. vesicularis , which contain mainly 2 mol of 2,3‐diamino‐2,3‐dideoxy‐D‐glucose and 1 mol of nonglycosidic phosphate as the backbone component and have partly different fatty acid compositions, were examined. TNF was induced by injecting various lipid A fractions into mice that had previously been sensitized with Mycobacterium bovis BCG vaccine. A major component of lipid A of both strains, referred to as A3 fraction, exhibited stronger TNF‐inducing activity than A2 fraction having incomplete acyl residues. The removal of ester‐linked fatty acyl groups by mild hydrazinolysis of the P. diminuta lipid A results in a marked decrease of the activity. These results suggest that the structure of the hydrophobic part, including the amide‐linked acyloxyacyl group(s), of the lipid A molecule play an important role in inducing TNF in the sera of mice.