Open AccessGENERATION OF INTRACELLULAR ACTIVE OXYGENS IN MOUSE FM3A CELLS BY 3‐HYDROXYAMINO‐1‐METHYL‐5 H ‐PYRIDO[4,3‐ b ]INDOLE, THE ACTIVATED TRP‐P‐2
Author(s) -
Wataya Yusuke,
Yamane Kazuko,
Hiramoto Kazuyuki,
Ohtsuka Yasuharu,
Okubata Yuko,
Negishi Kazuo,
Hayatsu Hikoya
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb00024.x
Subject(s) - formazan , intracellular , superoxide , indole test , metabolite , chemistry , biochemistry , microbiology and biotechnology , dna damage , stereochemistry , enzyme , biology , dna
Mouse FM3A cells in culture were treated with a reactive metabolite of 3‐amino‐1‐methyl‐5 H ‐pyrido[4,3‐ b ]indole (Trp‐P‐2), 3‐hydroxyamino‐1‐methyl ‐ 5 H ‐ pyrido[4,3 ‐ b ]indole (Trp ‐ P ‐ 2‐ (NHOH)). When the treated cells, which were judged as viable on the basis of trypan‐blue exclusion, were subjected to nitroblue tetrazolium staining, formazan was formed inside the cells, a fact suggesting the intracellular presence of superoxide. No formazan formation was detected on treatment of the cells with Trp‐P‐2. Single‐strand breaks in the cellular DNA took place during this treatment with Trp‐P‐2(NHOH). Since Trp‐P‐2(NHOH) in solution generates superoxide anion accompanying its oxidative degradation, we conclude that the Trp‐P‐2(NHOH) treatment produces intracellular active oxygens that can damage DNA.