Open AccessComparison of XAD‐2 Column and Blue Cotton Batch Techniques for Isolation of Metabolites of 2‐Amino‐3‐methylimidazo[4,5‐ f ]quinoline
Author(s) -
Inamasu Takeo,
Luks Howard Jay,
Weisburger John h.
Publication year - 1988
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1988.tb00009.x
Subject(s) - quinoline , isolation (microbiology) , chemistry , column (typography) , chromatography , column chromatography , biochemistry , biology , organic chemistry , microbiology and biotechnology , structural engineering , connection (principal bundle) , engineering
In studies on the metabolism of 2‐amino‐3‐methylimidazo[4,5‐ f ]quinoline (IQ) in the rat, two methods were used to concentrate IQ and its metabolites in urine: XAD‐2 columns and blue‐cotton extraction. These methods were compared as to the total recovery of *14 C‐label and the results of high‐performance liquid chromatography (HPLC) of the main metabolites. In the HPLC analysis, three major peaks in the polar region and two adjacent peaks in the nonpolar region having radioactivity were found in the urine from rats given *14 C‐IQ. XAD‐2 columns adsorbed approximately 65% and blue cotton 23% of the applied isotope from the urinary metabolites. Both methods efficiently adsorbed and totally released the relatively nonpolar compounds in a mixture of metabolites, including the compound administered, IQ. However, they failed to retain the more polar metabolites. Thus, both the XAD‐2 column and blue cotton adsorption techniques may be useful mainly to concentrate nonpolar compounds from larger volumes of aqueous solutions.