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Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine Sulfate) in the process of vaccine production
Author(s) -
Sakoda Yoshihiro,
Okamatsu Masatoshi,
Isoda Norikazu,
Yamamoto Naoki,
Ozaki Koichi,
Umeda Yasuto,
Aoyama Shigeyuki,
Kida Hiroshi
Publication year - 2012
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2012.00468.x
Subject(s) - sulfate , elution , virology , biology , ultrafiltration (renal) , chromatography , cellulose , hemagglutination , influenza vaccine , affinity chromatography , hemagglutination assay , virus , microbiology and biotechnology , chemistry , biochemistry , enzyme , organic chemistry , titer
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine Sulfate) was assessed for purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful for removing chicken egg‐derived impurities from allantoic fluids containing influenza viruses; the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that use of Cellufine Sulfate is a practical method for primary purification of influenza viruses in the process of influenza vaccine production.