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CpG‐enriched plasmid enhances the efficacy of the traditional foot‐and‐mouth disease killed vaccine
Author(s) -
Guo Xiaoyu,
Jia Hong,
Zhang Quan,
Yuan Weifeng,
Zhu Gang,
Xin Ting,
Zhu Hongfei,
Manuel SánchezVizcaíno José
Publication year - 2012
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2012.00438.x
Subject(s) - adjuvant , immunoadjuvant , vaccination , immune system , immunization , dna vaccination , virology , biology , cpg oligodeoxynucleotide , recombinant dna , plasmid , immunology , microbiology and biotechnology , dna , gene , dna methylation , biochemistry , gene expression , genetics
ABSTRACT A CpG‐enriched recombinant plasmid (pUC18‐CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil‐adjuvant vaccine after the initial inoculation. A subsequent vaccination‐challenge test showed an increased PD 50 value. Thus, coadministration of the recombinant plasmid with the oil‐adjuvant vaccine helped illicit an immune response earlier than that elicited by giving the vaccine alone. Our results showed that pUC18‐CpG can be a potent immunoadjuvant for the traditional FMD killed vaccine and can greatly enhance the traditional vaccine's efficacy when given in combination with it.