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Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010
Author(s) -
Ikeda Tatsuya,
Mizuta Katsumi,
Abiko Chieko,
Aoki Yoko,
Itagaki Tsutomu,
Katsushima Fumio,
Katsushima Yuriko,
Matsuzaki Yoko,
Fuji Naoko,
Imamura Tadatsugu,
Oshitani Hitoshi,
Noda Masahiro,
Kimura Hirokazu,
Ahiko Tadayuki
Publication year - 2012
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2012.00411.x
Subject(s) - enterovirus , isolation (microbiology) , biology , virus isolation , virology , epidemiology , molecular epidemiology , phylogenetic tree , respiratory system , polymerase chain reaction , microbiology and biotechnology , virus , genotype , medicine , gene , genetics , anatomy
To clarify the epidemiology of enterovirus 68 (EV68), which is one of the most rarely identified enteroviruses, virus isolation and molecular screening using RT‐PCR was performed on 6307 respiratory specimens collected at pediatric clinics in Yamagata, Japan between 2005 and 2010. In the years 2005–2009, 10, 1, 2, 0, and 2 (40) EV68‐positive cases, respectively, were identified by RT‐PCR. In 2010, 40 cases were identified altogether: 2 by isolation only, 26 by RT‐PCR only, and 12 by both isolation and RT‐PCR. Phylogenetic analysis indicated that plural genetically distinct clusters co‐circulated. These results suggest that that difficulty in EV68 isolation leads to an underestimation of the prevalence of EV68 infections.