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A novel anti‐peroxiredoxin autoantibody in patients with Kawasaki disease
Author(s) -
Fujieda Mikiya,
Karasawa Rie,
Takasugi Hisashi,
Yamamoto Masaki,
Kataoka Koichi,
Yudoh Kazuo,
Kato Tomohiro,
Ozaki Shoichi,
Wakiguchi Hiroshi
Publication year - 2012
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2011.00393.x
Subject(s) - autoantibody , antibody , immunology , kawasaki disease , vasculitis , systemic vasculitis , autoimmunity , peroxiredoxin , autoimmune disease , biology , oxidative stress , western blot , medicine , disease , enzyme , peroxidase , biochemistry , artery , gene
Antibodies to the anti‐oxidative peroxiredoxin (Prx) enzymes occur in both systemic autoimmune disease and vasculitis in adulthood. Because increased oxidative stress induces vasculitis in Kawasaki disease (KD), autoimmunity to Prxs in patients with KD was investigated. The presence of antibodies to Prx 1, 2 and 4 was analyzed by ELISA and Western blot. Of 30 patients with KD, 13 (43.3%) possessed antibodies to Prx 2, whereas these antibodies were present in only 1 of 10 patients (10.0%) with sepsis (4 with purulent meningitis and 6 with septicemia). In contrast, antibodies to Prx 1 and 4 were not detected in either group. There was no significant correlation among the titers of the three antibodies. Clinical parameters were compared between anti‐Prx 2‐positive and ‐negative patients. The presence of anti‐Prx 2 antibodies correlated with a longer period of fever and poor response to high‐dose γ‐globulin therapy in patients with KD. Anti‐Prx 2‐positive patients had significantly greater excretion of urinary 8‐isoprostaglandin than did anti‐Prx 2‐negative patients. These results provide the first evidence for an antibody to Prx 2 in patients with KD. They also suggest that this antibody might serve as a marker of disease severity and be involved in the pathophysiology of vasculitis in some patients with KD.