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Enumeration of viable Enterococcus faecalis , a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real‐time polymerase chain reaction
Author(s) -
Takahashi Yusuke,
Yoshida Akihiro,
Nagayoshi Masato,
Kitamura Chiaki,
Nishihara Tatsuji,
Awano Shuji,
Ansai Toshihiro
Publication year - 2011
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2011.00390.x
Subject(s) - propidium monoazide , enterococcus faecalis , enumeration , microbiology and biotechnology , biology , periodontitis , real time polymerase chain reaction , pathogen , bacteria , staphylococcus aureus , medicine , dentistry , biochemistry , genetics , mathematics , combinatorics , gene
To discriminate between viable and non‐viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real‐time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E . faecalis cells and permitted enumeration of both viable and non‐viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non‐viable E. faecalis cells were successfully discriminated in these clinical specimens. A real‐time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.