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Full sequence analysis of the original Sapporo virus
Author(s) -
Nakanishi Kaori,
Tatsumi Masatoshi,
KinoshitaNumata Kazuko,
Tsugawa Takeshi,
Nakata Shuji,
Tsutsumi Hiroyuki
Publication year - 2011
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2011.00358.x
Subject(s) - sapovirus , biology , virus , phylogenetic tree , virology , sequence analysis , nucleic acid sequence , whole genome sequencing , genetics , sequence (biology) , genome , gene , rotavirus
In this study, the full‐length genome sequence of the prototype of sapovirus, namely Sapporo virus (SV82), was identified. Sapporo virus RNA was extracted from a fecal sample, amplified by RT‐PCR and the PCR products sequenced directly and analyzed. Sequence analysis showed that Sapporo virus consists of 7433 nucleotides and has three open reading frames. The Sapporo strain shows 91.7% nucleotide sequence identity to the Manchester virus. Phylogenic analysis has also revealed the closeness of Sapporo virus to other sapovirus/genogroup I strains. Basic information on the evolutionary history of sapovirus analysis is provided here.