Premium
Strain‐specific detection by pulsed‐field gel electrophoresis of Lactobacillus gasseri TMC0356 in human feces after oral administration of these organisms
Author(s) -
Kawase Manabu,
He Fang,
Kubota Akira,
Miyazawa Kenji,
Yoda Kazutoyo,
Hiramatsu Masaru
Publication year - 2011
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2011.00350.x
Subject(s) - biology , lactobacillus gasseri , pulsed field gel electrophoresis , genomic dna , microbiology and biotechnology , restriction enzyme , genetics , strain (injury) , dna , lactobacillus , bacteria , genotype , gene , anatomy
The present study aimed to develop an innovative, strain‐specific means of identifying the probiotic Lactobacillus gasseri TMC0356 and to determine whether orally administered TMC0356 could be recovered from the human intestine. High molecular weight genomic DNA was isolated from TMC0356 and 14 reference strains of L. gasseri , including the type strain. The DNA samples were digested with the selected rare‐cutting restriction endonucleases Sma I, Sac II and Apa I and the resulting fragments separated by pulsed‐field gel electrophoresis (PFGE) in a size range between 20 to 290 kb. TMC0356 could be distinguished from the other L. gasseri strains on the basis of the Sma I and Sac II macrorestriction patterns. Furthermore, L. gasseri strains isolated from the feces of subjects who had ingested TMC0356 were identical to TMC0356 in the Sma I, Sac II and Apa I macrorestriction fragments of digested DNA. These results suggest that PFGE of genomic DNA digested with Sma I, Sac II, could be a practical means of identification of TMC0356. Furthermore, these results indicate that ingested TMC0356 can survive in, and colonize, the human intestine.