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Detection of norovirus, sapovirus, and human astrovirus in fecal specimens using a multiplex reverse transcription‐PCR with fluorescent dye‐labeled primers
Author(s) -
Shigemoto Naoki,
Fukuda Shinji,
Tanizawa Yukie,
Kuwayama Masaru,
Ohara Sachiko,
Seno Masato
Publication year - 2011
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2011.00325.x
Subject(s) - sapovirus , biology , norovirus , multiplex , virology , multiplex polymerase chain reaction , feces , microbiology and biotechnology , outbreak , fluorescence , polymerase chain reaction , gene , genetics , physics , quantum mechanics
We applied a multiplex reverse transcription‐PCR with fluorescent dye‐labeled primers (fluorescent multiplex RT‐PCR) for noroviruses (NoV), sapovirus (SaV), and human astrovirus (HAstV) to diagnose 71 outbreaks of acute gastroenteritis during July 2007 and May 2010 in Hiroshima prefecture. In this assay, the green, red, yellow, and blue fluorescence for NoV genogroup I, NoV genogroup II, SaV, and HAstV, respectively, were indicated on an agarose gel under ultraviolet light. In 61 virus‐positive outbreaks confirmed by fluorescent multiplex RT‐PCR, detection rates of outbreaks for NoVs, SaV, and HAstV were 96.7%, 3.3%, and 0%, respectively.