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Identification of cytosine‐phosphorothioate‐guanine oligodeoxynucleotide sequences that induce interferon‐γ production in feline immune cells
Author(s) -
Satoh Ryoichi,
Kotake Masako,
Takano Tomomi,
Motokawa Kenji,
Gemma Tsuyoshi,
Watanabe Rie,
Arai Setsuo,
Hohdatsu Tsutomu
Publication year - 2011
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00298.x
Subject(s) - cpg oligodeoxynucleotide , splenocyte , immune system , biology , adjuvant , cpg site , immunostimulant , peripheral blood mononuclear cell , microbiology and biotechnology , immunology , cats , in vitro , biochemistry , medicine , dna methylation , gene , gene expression
Unmethylated CpG‐ODN are known to enhance Th1‐type immune response. However, optimal sequences of CpG‐ODN for activating Th1‐type immune cells vary among species. It is necessary to identify the effective CpG‐ODN sequences in each species. In the present study, in order to identify the sequences of CpG‐ODN that produce fIFN‐γ in cats, 14 kinds of ODN were synthesized and examined regarding their ability to induce fIFN‐γ in feline PBMC and splenocytes. It was shown that some CpG‐ODN significantly induced fIFN‐γ production in splenocytes, but not in PBMC. We found that three kinds of CpG‐ODN (no. 2, 5′‐ggTGCATCGATGCAGggggG‐3′ ; no. 5, 5′‐ggTGCGTCGACGCAGggggG‐3′ ; no. 10, 5′‐ggTGCTACGTAGCAGggggG‐3′ ) specifically and significantly induced fIFN‐γ production in feline splenocytes. The reverse sequences, GpC‐ODN, do not cause significant fIFN‐γ production. The fIFN‐γ production inductivity of a mixture of CpG‐ODN nos. 2, 5 and 10 was higher than those of individual CpG‐ODN. When the CpG‐ODN mixture was encapsulated in an MCL and administrated to cats, the number of fIFN‐γ + cells in PBMC significantly increased. CpG‐ODN nos. 2, 5 and 10 should be useful to elicit a Th1‐type immune response as a vaccine adjuvant in cats.