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Development of species‐specific polymerase chain reaction primers for detection of Fusobacterium periodonticum
Author(s) -
Park SoonNang,
Park JaeYoon,
Kook JoongKi
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00279.x
Subject(s) - biology , amplicon , fusobacterium , polymerase chain reaction , primer (cosmetics) , bacteroidaceae , microbiology and biotechnology , subspecies , in silico pcr , bacteria , genetics , bacteroides , multiplex polymerase chain reaction , gene , paleontology , chemistry , organic chemistry
The purpose of this study was to develop species‐specific PCR primers for detection of Fusobacterium periodonticum . The specificity data showed that two sets of PCR primers, Fp‐F3/Fp‐R2 and Fp‐F1/Fp‐R2 PCR, produced amplicons from all the F . periodonticum , but not from the other species tested, which included 12 Fusobacterium species or subspecies and representative oral bacteria. The sensitivity of the primer sets was 4 or 40 pg of the chromosomal DNA from F . periodonticum ATCC 33693 T . These results suggest that these two sets of PCR primers are quite sensitive in detection of F . periodonticum in molecular epidemiological studies of periodontitis.