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Efficient propagation of progressive multifocal leukoencephalopathy‐type JC virus in COS‐7‐derived cell lines stably expressing Tat protein of human immunodeficiency virus type 1
Author(s) -
Nukuzuma Souichi,
Nakamichi Kazuo,
Kameoka Masanori,
Sugiura Shigeki,
Nukuzuma Chiyoko,
Miyoshi Isao,
Takegami Tsutomu
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00278.x
Subject(s) - progressive multifocal leukoencephalopathy , jc virus , virology , biology , cell culture , virus , leukoencephalopathy , dna , viral replication , genetics , medicine , disease , pathology
The high incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients compared with many other immunosuppressive diseases suggests that HIV‐1 infection is strictly related to the activation of JC virus (JCV) propagation. In this report, propagation of PML‐type JCV in COS‐7‐derived cell lines stably expressing HIV‐1 Tat (COS‐tat cells) has been examined. In COS‐tat cells, production of viral particles and replication of genomic DNA were markedly increased compared to COS‐7 cells, as judged by HA and real‐time PCR analyses. These results demonstrate that COS‐tat cells provide a useful model system for studying HIV‐1 Tat‐mediated propagation of PML‐type JCV.