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Low endotoxic activity of lipopolysaccharides isolated from Bradyrhizobium , Mesorhizobium , and Azospirillum strains
Author(s) -
Komaniecka Iwona,
Zdzisinska Barbara,
KandeferSzerszen Martyna,
Choma Adam
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00269.x
Subject(s) - bradyrhizobium , mesorhizobium , microbiology and biotechnology , biology , microbial inoculant , rhizobia , bradyrhizobium japonicum , lipopolysaccharide , rhizobiaceae , salmonella enterica , bacteria , salmonella , rhizobium , nitrogen fixation , symbiosis , immunology , genetics
The endotoxic activities of lipopolysaccharides (LPS) isolated from different strains of rhizobia and rhizobacteria ( Bradyrhizobium , Mesorhizobium , and Azospirillum ) were compared to those of Salmonella enterica sv. Typhimurium LPS. The biological activity of all the examined preparations, measured as Limulus lysate gelation, production of tumor necrosis factor (TNF), interleukin‐1β (IL‐1β), and interleukin‐6 (IL‐6), and nitrogen oxide (NO) induction in human myelomonocytic cells (line THP‐1), was considerably lower than that of the reference enterobacterial endotoxin. Among the rhizobial lipopolysaccharides, the activities of Mesorhizobium huakuii and Azospirillum lipoferum LPSs were higher than those of the LPS preparations from five strains of Bradyrhizobium . The weak endotoxic activity of the examined preparations was correlated with differences in lipid A structure compared to Salmonella .