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Conversion of viable but nonculturable Vibrio cholerae to the culturable state by co‐culture with eukaryotic cells
Author(s) -
Senoh Mitsutoshi,
GhoshBanerjee Jayeeta,
Ramamurthy Thandavarayan,
Hamabata Takashi,
Kurakawa Takashi,
Takeda Makoto,
Colwell Rita R.,
Nair G. Balakrish,
Takeda Yoshifumi
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00245.x
Subject(s) - vibrio cholerae , viable but nonculturable , incubation , microbiology and biotechnology , vibrionaceae , biology , cell culture , hela , cholera , incubation period , bacteria , biochemistry , genetics
VBNC Vibrio cholerae O139 VC‐280 obtained by incubation in 1% solution of artificial sea water IO at 4°C for 74 days converted to the culturable state when co‐cultured with CHO cells. Other eukaryotic cell lines, including HT‐29, Caco‐2, T84, HeLa, and Intestine 407, also supported conversion of VBNC cells to the culturable state. Conversion of VBNC V. cholerae O1 N16961 and V. cholerae O139 VC‐280/pG13 to the culturable state, under the same conditions, was also confirmed. When VBNC V. cholerae O139 VC‐280 was incubated in 1% IO at 4°C for up to 91 days, the number of cells converted by co‐culture with CHO cells declined with each additional day of incubation and after 91 days conversion was not observed.