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Localization by scanning immunoelectron microscopy of triosephosphate isomerase, the molecules responsible for contact‐mediated killing of Cryptococcus , on the surface of Staphylococcus
Author(s) -
Yamaguchi Masashi,
Ikeda Reiko,
Nishimura Masako,
Kawamoto Susumu
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00225.x
Subject(s) - immunoelectron microscopy , staphylococcus aureus , biology , triosephosphate isomerase , microbiology and biotechnology , protein a , cytoplasm , ultrastructure , biochemistry , antibody , bacteria , enzyme , immunology , anatomy , genetics
In our previous studies, TPI were found to be the molecules responsible for contact‐killing of C. neoformans by S. aureus cells. Since TPI is a glycolytic protein that functions in the cytoplasm, evidence that TPI is present on the surface of S . aureus was required. In the present study, the presence of TPI on the cell surface of S . aureus was demonstrated by agglutination test and scanning immunoelectron microscopy. Furthermore, TPI was found to be present at a lower density than protein A/G molecules on the surface of S . aureus .