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A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing
Author(s) -
Umeda Kaoru,
Seto Yoshiyuki,
Kohda Tomoko,
Mukamoto Masafumi,
Kozaki Shunji
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00213.x
Subject(s) - biology , multiplex polymerase chain reaction , clostridium botulinum , multiplex , typing , neurotoxin , gene , gene cluster , computational biology , clostridiaceae , genetics , microbiology and biotechnology , polymerase chain reaction , toxin , biochemistry
A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype ( boNT/A1 or / A2 ) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986–1987 or 1999–2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.