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Classification of perA sequences and their correlation with autoaggregation in typical enteropathogenic Escherichia coli isolates collected in Japan and Thailand
Author(s) -
Iida Mariko,
Okamura Noboru,
Yamazaki Mitsugu,
Yatsuyanagi Jun,
Kurazono Takayuki,
Suzuki Rieko,
Hiruta Noriaki,
Isobe Junko,
Seto Kazuko,
Kawano Kimiko,
Narimatsu Hiroshi,
Ratchtrachenchai OrnAg,
Okabe Nobuhiko,
Ito Kenitiro
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00212.x
Subject(s) - biology , microbiology and biotechnology , enteropathogenic escherichia coli , frameshift mutation , el tor , pera , escherichia coli , virulence , plasmid , gene , mutation , genetics , vibrio cholerae , bacteria , horticulture , orange (colour)
Enteropathogenic Escherichia coli (EPEC) strains produce a bundle‐forming pilus (BFP) that mediates localized adherence (LA) to intestinal epithelial cells. The major structural subunit of the BFP is bundlin, which is encoded by the bfpA gene located on a large EAF plasmid. The perA gene has been shown to activate genes within the bfp operon. We analyzed perA gene polymorphism among typical ( eae ‐ and bfpA ‐ positive) EPEC strains isolated from healthy and diarrheal persons in Japan ( n = 27) and Thailand ( n = 26) during the period 1995 to 2007 and compared this with virulence and phenotypic characteristics. Eight genotypes of perA were identified by heteroduplex mobility assay (HMA). The strains isolated in Thailand showed strong autoaggregation and had an intact perA , while most of those isolated in Japan showed weak or no autoaggregation, and had a truncated perA due to frameshift mutation. The degree of autoaggregation was well correlated with adherence to HEp‐2 cells, contact hemolysis and BFP expression. Our results showed that functional deficiency due to frameshift mutation and subsequent nonsense mutation in perA reduced BFP expression in typical EPEC strains isolated in Japan.

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