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Presence of multiple copies of capsulation loci in invasive Haemophilus influenzae type b (Hib) strains in Japan before introduction of the Hib conjugate vaccine
Author(s) -
Ueno Kentaro,
Nishi Junichiro,
Imuta Naoko,
Tokuda Koichi,
Kawano Yoshifumi
Publication year - 2010
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00196.x
Subject(s) - hib vaccine , locus (genetics) , biology , conjugate vaccine , virology , genetics , pasteurellaceae , haemophilus influenzae , southern blot , microbiology and biotechnology , gene , immunization , bacteria , antibody
Despite the effectiveness of the Hib vaccine, multiple amplification of the cap b locus contributes to vaccine failure. However, there has been no report on the effect of Hib locus amplification in Japan. We examined 24 Hib strains from Japanese children with invasive diseases due to Hib. Although all strains showed the same cap b sequence, Southern blot analysis showed that four strains (16.7%) harbored multiple copies (more than two) of the cap b locus. Careful analysis of the locus in circulating Hib strains is necessary now that the Hib vaccine has been introduced into Japan.