Premium
A new antimicrobial peptide isolated from Oudneya africana seeds
Author(s) -
Hammami Riadh,
Hamida Jeannette Ben,
Vergoten Gérard,
Lacroix JeanMarie,
Slomianny MarieChristine,
Mohamed Neffati,
Fliss Ismail
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00183.x
Subject(s) - biology , antimicrobial , ampicillin , chloramphenicol , microbiology and biotechnology , antibiotics , listeria monocytogenes , escherichia coli , bacteria , biochemistry , genetics , gene
Oudneya africana R. Br. (Brassicaceae), a wild‐growing plant in the arid region of Tunisia, is used in ethno‐medicinal treatment of microbial infections. Validation of ethno‐therapeutic claims pertaining to the plant was sought by investigating its antimicrobial activity. A proteinaceous extract of the seeds, called AS‐3000, showed activity against various organisms including L. monocytogenes , E. coli , B. subtilis , E. hirae , P. aeruginosa , S. aureus and C. albicans . Extract AS‐3000 exhibited a synergistic effect against L. ivanovii when combined with vancomycin or chloramphenicol. The post‐antibiotic inhibitory effect of the ampicillin/AS‐3000 combination was 2.3‐fold greater than for the antibiotic alone. The mode of action of AS‐3000 on Listeria and Escherichia was visible using SEM. These results support the use of O. africana for treating microbial infections.