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Isolation and characterization of bacteriophages specific for Campylobacter jejuni
Author(s) -
Hwang Sunyoung,
Yun Jiae,
Kim KwangPyo,
Heu Sunggi,
Lee Seungdon,
Ryu Sangryeol
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00163.x
Subject(s) - campylobacter jejuni , biology , isolation (microbiology) , microbiology and biotechnology , campylobacter , virology , bacteria , genetics
Human infection by Campylobacter jejuni is mainly through the consumption of contaminated poultry products, which results in gastroenteritis and, rarely, bacteremia and polyneuropathies. In this study, six C. jejuni ‐specific bacteriophages (CPS1–6) were isolated by the spot‐on‐the‐lawn technique from chicken samples in Korea and characterized for potential use as biocontrol agents. All isolated bacteriophages exhibited a high specificity, being able to lyse only C. jejuni , but not other Gram–negative bacteria, including C. coli , Escherichia coli , Salmonella spp., and Gram–positive bacteria. Bacteriophages contain an icosahedral head and a contractile tail sheath in transmission electron microscopy, and possess ds‐DNA with an average genome size of approximately 145 kb; therefore, all bacteriophages are categorized into the Myoviridae family. Bacterial lysis studies in liquid media revealed that CPS2 could be used to control the growth of C. jejuni .