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Inhibitory effect of serotonin antagonists on JC virus propagation in a carrier culture of human neuroblastoma cells
Author(s) -
Nukuzuma Souichi,
Nakamichi Kazuo,
Nukuzuma Chiyoko,
Takegami Tsutomu
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00156.x
Subject(s) - biology , jc virus , progressive multifocal leukoencephalopathy , ketanserin , 5 ht receptor , ritanserin , cell culture , virology , receptor , virus , microbiology and biotechnology , cancer research , serotonin , genetics
Human polyomavirus, JCV, causes fatal demyelinating disease, progressive multifocal leukoencephalopathy (PML). It has been shown that 5HT 2A R acts as a cellular receptor for JCV on human glial cells. In the current study, we examined the inhibitory effects of 5HT 2A R antagonists, ketanserin and ritanserin, both on JCV infection and on propagation by using human neuroblastoma cells IMR‐32 and JCI, which continuously produce JCV. Transcriptional analysis revealed that 5HT 2A R was constitutively expressed in JCI cells. Treatments with 5HT 2A R antagonists led to a significant reduction in the titers of progeny viruses and the population of infected JCI cells. In addition, the amount of JCV genomic DNA was decreased in JCI cells in the presence of 5HT 2A R antagonists. These results indicate that 5HT 2A R antagonists have an inhibitory effect on JCV infection and reproduction, and JCI cells are applicable to an experimental model for pharmacological evaluation of antiviral agents against JCV.