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Effect of GM‐CSF on cytokine induction by soluble β‐glucan SCG in vitro in β‐glucan‐treated mice
Author(s) -
Hida Toshie H.,
Kawaminami Hiromi,
Ishibashi Kenichi,
Miura Noriko N.,
Adachi Yoshiyuki,
Yadomae Toshiro,
Ohno Naohito
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00139.x
Subject(s) - in vitro , splenocyte , cytokine , biology , glucan , spleen , in vivo , receptor , microbiology and biotechnology , immunology , biochemistry
SCG is a 6‐branched 1,3‐β‐ d ‐glucan, which are major cell wall structural components in fungi. Leukocytes from DBA/1 and DBA/2 mice are highly sensitive to SCG, producing cytokines such as GM‐CSF, IFN‐γ, TNF‐α and IL‐12p70, but not IL‐6. GM‐CSF plays a key biological role in this activity. In the present study, we examined the effect of giving i.p. SCG to DBA/2 mice on cytokine production in vitro . SCG was given i.p. to DBA/2 mice on day 0. Splenocytes were prepared on day 7 and cultured in the presence of SCG in vitro . The levels of cytokine production induced by SCG in vitro were lower in the cells from SCG‐treated mice than in control mice. Expression of the β‐glucan receptor, dectin‐1, in SCG‐treated mice was comparable with that shown in control mice. However, the consumption of exogenously added rmGM‐CSF in vitro was observed in SCG‐treated mice. The addition of a large amount of rmGM‐CSF to the culture medium resulted in larger amounts of TNF‐α and IL‐6 in SCG‐treated mice than in normal mice. These results suggested that GM‐CSF was closely related with the reactivity of β‐glucan. Giving SCG increased the number of macrophages and granulocytes in the spleen. These results suggested that in SCG‐treated mice, a change of cell population would be related to modulation of the profile of cytokine production induced by SCG in vitro .