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Improved cellular immune response elicited by a ubiquitin‐fused ESAT‐6 DNA vaccine against Mycobacterium tuberculosis
Author(s) -
Wang Qingmin,
Kang Lin,
Wang Xiaohua
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00138.x
Subject(s) - esat 6 , dna vaccination , immune system , tuberculosis vaccines , biology , mycobacterium tuberculosis , virology , antigen , immunology , microbiology and biotechnology , tuberculosis , immunization , medicine , pathology
The present study evaluated the immune response elicited by a ubiquitin‐fused ESAT‐6 DNA vaccine against Mycobacterium tuberculosis. BALB/c mice were vaccinated with plasmid DNA encoding ESAT‐6 protein, ubiquitin‐fused ESAT‐6 DNA vaccine (UbGR‐ESAT‐6), pcDNA3‐ubiquitin and blank vector, respectively. ESAT‐6 DNA vaccine immunization induced a Thl‐polarized immune response. The production of Thl‐type cytokine (IFN‐γ) and proliferative T‐cell responses was enhanced significantly in mice immunized with UbGR‐ESAT‐6 fusion DNA vaccine, compared to non‐fusion DNA vaccine. This fusion DNA vaccine also resulted in an increased relative ratio of IgG 2a to IgG l and the cytotoxicity of T cells. Thus, the present study demonstrated that the UbGR‐ESAT‐6 fusion DNA vaccine inoculation improved antigen‐specific cellular immune responses, which is helpful for protection against tuberculosis infection.