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Interaction between superantigen and T‐cell receptor Vβ element determines levels of superantigen‐dependent cell‐mediated cytotoxicity of CD8 + T cells in induction and effector phases
Author(s) -
Li ZhongJuan,
Omoe Katsuhiko,
Shinagawa Kunihiro,
Yagi Junji,
Imanishi Ken’ichi
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00136.x
Subject(s) - superantigen , t cell receptor , biology , cytotoxic t cell , cd8 , t cell , microbiology and biotechnology , t lymphocyte , effector , antigen , immunology , immune system , in vitro , biochemistry
Specific superantigens activate different T‐cell fractions with distinct TCR Vβ elements in association with MHC class II molecules and also induce SDCC against MHC class II + target cells. In the present study, to determine whether the responsiveness of each CD8 + T‐cell fraction expressing a different TCR Vβ element is primarily determined by the TCR Vβ, we compared the levels of proliferation and SDCC in Vβ3 + and Vβ11 + T cells upon stimulation with SEA. Upon stimulation with SEA wt , the levels of proliferation were higher in Vβ3 + T cells than in Vβ11 + T cells. The levels of SDCC were also higher for the combination of Vβ3 + T cells and SEA wt than for the combination of Vβ11 + T cells and SEA wt during both the induction phase and the effector phase. In addition, upon stimulation with SEA m , the levels of proliferation were higher in Vβ11 + T cells than in Vβ3 + T cells. And then, the levels of SDCC were also higher for the combination of Vβ11 + T cells and SEA m than for the combination of Vβ3 + T cells and SEA m during both the induction phase and the effector phase. These results suggest that the SAG‐responsiveness of each CD8 + T‐cell fraction expressing a different TCR Vβ element is primarily determined by the interaction between the TCR Vβ element and the SAG.