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Screen of multifunctional monoclonal antibodies against hepatitis B core virus‐like particles
Author(s) -
Sun Chang,
Ding FeiXiang,
Wang Fang,
He XiaoWen,
He Ying,
Li ZhaoShen,
Sun ShuHan
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00135.x
Subject(s) - immunogen , epitope , monoclonal antibody , virology , virus like particle , biology , immunofluorescence , virus , capsid , conformational epitope , western blot , antigen , antibody , hepatitis b virus , cell fusion , microbiology and biotechnology , cell culture , recombinant dna , immunology , biochemistry , gene , genetics
HBc‐VLP can be used in an epitope presentation system to carry foreign epitopes and mimic live virus in order to study viral particle uptake, virion‐mediated activation and antigen presentation by dendritic cells. In this study, a multifunctional mAb was produced using a novel research strategy. A truncated HBc‐VLP bone vector with a special conformation was used as an immunogen and the target hybridoma cell lines were screened by a series of tests; including ELISA, Western blot, and cellular immunofluorescence based on the epitope presentation system. The screened monoclonal antibody was used to identify the HBc‐VLP vector, a fusion HBc‐VLP vaccine, and intracellular HBV capsids. The new strategy facilitated acquisition of the desired mAbs and will serve as a reference for other VLP‐related research.