Premium
Suppression of anti‐ Candida activity of macrophages by a quorum‐sensing molecule, farnesol, through induction of oxidative stress
Author(s) -
Abe Shigeru,
Tsunashima Rumi,
Iijima Ryosuke,
Yamada Tsuyoshi,
Maruyama Naho,
Hisajima Tatsuya,
Abe Yoshie,
Oshima Haruyuki,
Yamazaki Masatoshi
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2009.00128.x
Subject(s) - farnesol , candida albicans , biology , trolox , apoptosis , corpus albicans , viability assay , intracellular , microbiology and biotechnology , oxidative stress , biochemistry , antioxidant capacity
Farnesol is well known as a quorum‐sensing molecule of Candida albicans . To assess the pathological function of farnesol, its effects on macrophage viability and functions including growth inhibitory activities against C. albicans were examined in vitro . Murine macrophages, when cultured in the presence of 56–112 μM of farnesol for 1–2 hr, decreased their activity inhibiting the mycelial growth of C. albicans and lost their viability. This suppression of macrophage function by farnesol was neutralized by the coexistence of the anti‐oxidants probucol and trolox. Macrophages cultured in the presence of farnesol for 2 hr displayed morphological change of nuclei and DNA fragmentation, which suggested apoptosis of the cells. Intracellular production of ROS in the farnesol‐treated macrophages was shown by fluorescence of DCFH‐DA and increase of peroxidized materials. These effects of farnesol were blocked by probucol or trolox. These results indicate that farnesol lowered viability of the murine macrophages and suppressed their anti‐ Candida activity, perhaps through induction of ROS.