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New ITS genotype of Cryptococcus gattii isolated from an AIDS patient in Brazil
Author(s) -
Kang Yingqian,
Tanaka Hiroko,
Moretti Maria Luiza,
Mikami Yuzuru
Publication year - 2009
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00101.x
Subject(s) - cryptococcus gattii , biology , genotype , cryptococcus , virology , cryptococcosis , microbiology and biotechnology , genetics , gene
Based on combinations of nine variable nucleotides at nine different base positions in the ITS1‐5.8S‐ITS2 region, Cryptococcus gattii strains were classified into six genotypes. A new genotype of C. gattii , designated as ITS type 8, was isolated from an AIDS patient in Brazil. The ITS type 8 strain is closely related to the ITS type 4 strain, which has been frequently isolated in Brazil and the USA, but which shows ITS‐signatured nucleotide difference at each nucleotide position. The ITS type 8 strain is also differentiated from all heretofore reported ITS types of C. gattii strains in the RAPD band patterns and IGS sequence information.